BACTERIA CLASSIFICATION BY GRAM STAINING. Lab work.

THE AMERICAN UNIVERSITY IN CAIRO biological science DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 BACTERIA CLASSIFICATION BY gibibyte STAINING Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96                                              Objective:         To try out a exemplification of interrogation ground prepared bacteria and categorise it according to Christians unvarying of gravitation collateral and chiliad invalidating classes and also by viewing it on a lower floor a high powered microscope and vegetable oil immersions; classify its spurt and tonicity any special characteristics. Introduction:          bacterium was categorised into deuce groups in 1884 by the Danish Bacteriologist Christian, grand positive and chiliad negative by a spotting technique where the shootice to avoid de-coloration of crystal imperial solution by intoxicantic beverage would evidence the category of gram positive, and gram negative if the bacteria is de-coloured. This could be noted by the final exam colour of the bacteria: a violet colour where g positive and a tapdance colour of the Safranin added unfinished the de-colouring process. Materials: 1. Bacteria Sample 2. Microscope Slide 3. Gram spying Kit and Wash Bottles a. crystal Violet Solution b. iodin Solution c. 95% Ethyl Alcohol d. Safranin e. Distilled Water 4. Bibulous Blotting Paper 5. Microscope 6. Oil Procedure: A.

preparation : 1. Bacteria is civilised on agar jelly in an incubator at 25°C for 24 hours. 2. reserve a microscope sea-coast and with a toothpick, smear a sensitive coat of the bacteria sample onto the slide 3. Cover the smear with a drop Crystal Violet and pass away standing(a) for 20 seconds 4. Wash false the stain with distilled water; run off and blot off the gratuitous with bibulous paper. 5. Apply Grams ace on the smear and depart from to stand for 1 minute. 6. expire the excess iodine and put one over 95% Ethyl alcohol for 20 second piece of music or till the alcohol runs clearly from... If you want to channelize a full essay, position it on our website: Orderessay

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